This sequence is only available under the Restricted Use Terms. This prohibits publications that use this sequence as focal data during the restricted-use period, except with the consent of the sequence submitters.
Canada/PP_006XDHK.6/2026-05-15
Jonathan Audet, Mable Chan, Dave Safronetz, John Tyson, Tracy Lee, Branco Cheung, Natalie Prystajecky, Eric Eckbo, Muhammad Morshed, Agatha Jassem & Linda Hoang
Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada; BC Center for Disease Control Public Health Laboratory
This sequence is specific to Illumina generated sequence data, but Oxford Nanopore sequence data was also generated and showed only 2 additional mixed base positions across all segments.
Sequencing protocol
The RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen). The reverse transcription was performed using SuperScript IV (ThermoFisher) following manufacturers recommendations, using 8 µL of RNA extract. A two pool 400bp tiled amplicon scheme was designed using PrimalScheme with PP_006WBLH.2 as the reference genome ( https://labs.primalscheme.com/detail/bccdc-andv-2026/400/v1....