V. P. Martinez, U. Perez-Sautu, D. O. Alonso, N. Di Paola, C. M. Bellomo, A. A. Iglesias, R. M. Coelho, N. Periolo, E. R. Nagle, J. A. Chitty, C. B. Pratt, M. R. Wiley, J. Diaz, E. Biondo, L. Lewis, C. Anselmo, F. Pontoriero, E. Lavarra, J. H. Kuhn, M. Sanchez-Lockhart, A. Edelstein, D. Cisterna, J. Campos, M. Kaler, ..., C. Pratt
United States Army Medical Research Institute for Infectious Diseases USAMRIID, Center for Genome Sciences
Hospital Administrator at the Epuyen Rural Hospital
Sequencing
Purpose of sequencing
Outbreak
Sequencing protocol
RNA was extracted from 400 µl of whole blood in TRIzol LS reagent using the TRIzol™ Reagent and Phasemaker™ Tubes Complete System (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s protocol. RNA sequencing libraries were prepared using the KAPA RNA HyperPrep kit (KAPA Biosystems, Wilmington, MA, USA) following the manufacturer’s guidelines. Unique dual-index...