Restricted-Use sequence

This sequence is only available under the Restricted Use Terms. This prohibits publications that use this sequence as focal data during the restricted-use period, except with the consent of the sequence submitters.
Democratic_Republic_of_the_Congo/PP_003RXFM.2/2025-09
Adrienne Amuri-Aziza, Gradi Luakanda - Ndelemo, Jean-Claude Makangara-Cigolo, Prince Akil-Bandali, Princesse Paku-Tshambu, Sam Wilkinson, Louis Tshulo, Josh Quick, Andre Citenga, Chloe Muswamba-Kayembe, Olga Ntumba-Tshitenge, Emmanuel Lokilo-Lofiko, Fiston Cikaya-Kankolongo, Ola Rilia, Servet Kimbonza, Elisabeth Pukuta, Daniel Mukadi, Christian Ngandu, Mathias Mossoko, Gabriel Kabamba -Lungenyi, Raphael Lumembe-Numbi, Elzedeck Mabika-Bope, Patrick Mukadi, Catherine Pratt, ..., Placide Mbala-Kingebeni

Institut National de Recherche Biomédicale

Sample details

Collection date
2025-09
Sample received date
2025-09-03
Sampling location
Democratic Republic of the Congo (Kasai)
Isolate name
25FHV170

Data use terms

Data use terms
RESTRICTED

Outbreak

Outbreak
Ebov-2025

Host

Host
Homo sapiens

Sequencing

Sequencing date
2025-09-03
Amplicon PCR primer scheme
https://labs.primalscheme.com/detail/ebola-mangina-2018/400/v3.0.0/?q=ebola
Amplicon size
400
Sequencing instrument
Gridion
Sequencing protocol
amplicon sequencing
Sequenced by - organization
Institut National de Recherche Biomédicale
Sequenced by - contact email
labgenpath@inrb.cd
Consensus sequence software name
https://artic.network/amplicon-nf/amplicon-nf-epi2me-sop.html
Consensus sequence software version
0.4.6

Submission details

Submission ID
25FHV170
Submitting group
Date submitted
2025-09-10 20:49:32 UTC
Date released
2025-09-10 20:51:35 UTC
Earliest release date
2025-09-05

Alignment and QC

Length
18754 (95.8%)
# of SNPs
85
# of deleted bases
1
# of unknown bases
588

Nucleotide mutations

Mutations called relative to the NC_002549.1 reference

Substitutions

  • T221C
  • C287T
  • G381A
  • A388G
  • C420T
  • C464T
  • A619G
  • T764C
  • C1198T
  • A1471G
  • G1996A
  • T2019C
  • G2023A
  • G2032A
  • C2101T
  • G2242A
  • A3738G
  • T4565C
Deletions

18472
Insertions
N/A

Amino acid substitutions

Substitutions called relative to the NC_002549.1 reference

Substitutions

GP

  • GP:D338N
  • GP:I544T

L

  • L:V79I
  • L:S479G
  • L:K937R
  • L:R1125K
  • L:M1695V
  • L:I2137V

NP

  • NP:I517T

VP24

  • VP24:M212K

VP35

  • VP35:N204D

VP40

  • VP40:I54T
  • VP40:V298A
Deletions
N/A
Insertions
N/A

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