PP_003RSKH.3

Restricted sequence

This sequence is only available under the Restricted Use Terms. If you make use of this data, you must follow the terms of use.
Display Name: Italy/PP_003RSKH.3/2024-09-11
Simone Malago', Concetta Castilletti, Chiara Piubelli, Michela Deiana, Antonio Mori, Francesca Greco, Maria Vittoria Mauro & Antonio Mastroianni

Sample details

Collection date
2024-09-11
Collection date (lower bound)
2024-09-11
Collection date (upper bound)
2024-09-11
Sample received date
2025-07-15
Collection subdivision level 1
Calabria
Collection country
Italy

Data use terms

Data use terms
RESTRICTED

Data use terms history

ChangedUserData use terms
2025-09-04 09:32simomalaRESTRICTED until 2025-11-30
Data use terms URL

Lineage

Lineage
2

Authors

Author affiliations
Department of infectious tropical diseases and microbiology, IRCCS Sacro Cuore Don Calabria hospital, Negrar di Valpolicella, Italy; Department of infectious tropical diseases and microbiology, IRCCS Sacro Cuore Don Calabria hospital, Negrar di Valpolicella, Italy; Department of infectious tropical diseases and microbiology, IRCCS Sacro Cuore Don Calabria hospital,...

Sampling

Body product
Urine

Host

Host taxon ID
Host name - scientific
Homo sapiens
Host age
71

Sequencing

Sequencing date
2025-08-11
Sequencing instrument
Illumina NextSeq 1000
Sequencing protocol
Genomes were generated through hybrid-capture sequencing through VSP enrichment panel (Illumina). Libraries were created following the RNA prep with enrichment (Illumina). Data was produced using P1 flowcell (300 cycles) in 2x150 configuration.
Consensus sequence software name
SPAdes
Consensus sequence software version
3.11.1
Depth of coverage
773
Breadth of coverage
100

Alignment and QC metrics

Total stop codons
0
Length
11022
Total SNPs
2226
Total inserted nucs
38
Total deleted nucs
17
Total ambiguous nucs
1
Total unknown nucs
0
Total frame shifts
0
Completeness
99.74%

Submission details

Submission ID
02-2025_SCDC
Submitting group
Date submitted
2025-09-04 09:35:19 UTC
Date released
2025-09-04 09:36:07 UTC
Earliest release date
2025-09-01

Nucleotide mutations

Mutations called relative to the NC_009942.1 reference

Substitutions

  • C123T
  • G126A
  • G128A
  • A156T
  • T167G
  • G168A
  • T180A
  • T199C
  • C204T
  • C210T
  • C216G
  • G219A
  • G222C
  • A231T
  • T234C
  • C246T
  • C255T
  • G261A
    • Deletions
    6994-6996, 10467-10470, 10487-10494, 10904, 10921
    Insertions
    ins_7006:TTGAGA, ins_10408:TGTATTGAGT, ins_10417:GTTGTAGTGTTTA, ins_10452:AGA, ins_10461:AG, ins_10483:GAT, ins_10501:T

    Amino acid mutations

    Mutations called relative to the NC_009942.1 reference

    Substitutions

    2K

  • 2K:M15L

    • NS1

  • NS1:S9G
  • NS1:Y34F
  • NS1:K44R
  • NS1:K51A
  • NS1:V71I
  • NS1:E94N
  • NS1:S99A
  • NS1:T105A
  • NS1:I113M
  • NS1:L123I
  • NS1:V135I
  • NS1:K141E
  • NS1:Q146A
  • NS1:L153M
  • NS1:K170R
  • NS1:V171I
  • NS1:S174T
  • NS1:L192M

    • NS2A

  • NS2A:M34I
  • NS2A:L38M
  • NS2A:I39L
  • NS2A:S68A
  • NS2A:M90L
  • NS2A:N104S
  • NS2A:V112A
  • NS2A:H119Y
  • NS2A:R122K
  • NS2A:Q123N
  • NS2A:I124V
  • NS2A:L126S
  • NS2A:I129V
  • NS2A:A137S
  • NS2A:T147S
  • NS2A:T150N
  • NS2A:R167K
  • NS2A:I183V

    • NS2B

  • NS2B:A41V
  • NS2B:S61T
  • NS2B:M88I
  • NS2B:V103A
  • NS2B:V119I
  • NS2B:V120I

    • NS3

  • NS3:K11R
  • NS3:Q92H
  • NS3:F130Y
  • NS3:D172E
  • NS3:I175A
  • NS3:R203K
  • NS3:R215K
  • NS3:A233S
  • NS3:N253S
  • NS3:V283I
  • NS3:S302A
  • NS3:S331A
  • NS3:L336M
  • NS3:S347T
  • NS3:T356V
  • NS3:V384I
  • NS3:T436E
  • NS3:E439D

    • NS4A

  • NS4A:I6V
  • NS4A:K11R
  • NS4A:T64S
  • NS4A:M65L
  • NS4A:A85V
  • NS4A:V86I
  • NS4A:V89A

    • NS4B

  • NS4B:S11N
  • NS4B:S14G
  • NS4B:F17L
  • NS4B:Q19H
  • NS4B:R20K
  • NS4B:I21P
  • NS4B:V23T
  • NS4B:K24R
  • NS4B:N26T
  • NS4B:F27T
  • NS4B:S28L
  • NS4B:M29G
  • NS4B:G30V
  • NS4B:T48A
  • NS4B:T116A
  • NS4B:I168V
  • NS4B:L183M
  • NS4B:K193R
  • NS4B:I202T

    • NS5

  • NS5:Q18H
  • NS5:I33T
  • NS5:K45R
  • NS5:V49I
  • NS5:I78V
  • NS5:R101K
  • NS5:C139A
  • NS5:C140S
  • NS5:I162V
  • NS5:R177K
  • NS5:V181I
  • NS5:K190R
  • NS5:L197T
  • NS5:R224H
  • NS5:V229I
  • NS5:R247K
  • NS5:Y254F
  • NS5:R287K

    • capsid

  • capsid:S11N
  • capsid:V24G
  • capsid:S100T
  • capsid:K108T
  • capsid:T109A
  • capsid:I111F
  • capsid:A112T
  • capsid:V113I
  • capsid:M114L
  • capsid:I115L
  • capsid:S120C
  • capsid:V121A

    • env

  • env:E55D
  • env:T64S
  • env:K71R
  • env:D83E
  • env:R93K
  • env:S122T
  • env:I126T
  • env:R128W
  • env:T129I
  • env:L131Q
  • env:V159T
  • env:L167F
  • env:A172S
  • env:N199S
  • env:T205S
  • env:T208A
  • env:T210S
  • env:V232T

    • prM

  • prM:M44L
  • prM:D46E
  • prM:S57A
  • prM:A73S
  • prM:M112L
  • prM:V156A
  • prM:V157I
    • Deletions
    None
    Insertions
    ins_NS4B:31:N

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