This sequence is only available under the Restricted Use Terms. This prohibits publications that use this sequence as focal data during the restricted-use period, except with the consent of the sequence submitters.
Netherlands/Run_1321_BC89/2026-05-22
B. B. Oude Munnink, D. F. Nieuwenhuijse, S. C. van den Boom, L. Zaeck, M. Boter, H. Cassidy, L. Schuele, R. Molenkamp, A. van der Eijk, C. GeurtsvanKessel & M. Koopmans
Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands
The design comprised 62 overlapping primers generating ~400 bp amplicons in two pools. A third pool contained three L-segment amplicons targeting outbreak-specific SNPs to increase sensitivity for outbreak specimens. Random priming used 65°C for 5 min followed by 2 min snap cooling at 4°C. cDNA was synthesized with SuperScript IV (Invitrogen, Carlsbad, USA) at 23°C for 10 min,...
Sequenced by - organization
Erasmus MC
Consensus sequence software name
ARTIC field bioinformatics pipeline (https://github.com/artic-network/fieldbioinformatics)