This sequence is only available under the Restricted Use Terms. This prohibits publications that use this sequence as focal data during the restricted-use period, except with the consent of the sequence submitters.
Netherlands/Run_1313_BC05/2026-05-18
Janko van Beek, Saskia A. Rutjes, Michele van Vugt, Jans Velzing, Kaïn S. Saygan, Erley Lizarazo Forero, Hayley Cassidy, Gabriel Goderski, Bas van der Veer, Dennis de Meulder, Putri Ayu Fajar, Zsofia Igloi, Roy van Hattum, Ettore Severi, Freddy Banza-Mutoka, Leonard Schuele, David Nieuwenhuijse, Bas B. Oude Munnink, Alvin Bartels, Thijs Veenstra, Mariëlle Dirven, Reinoud Wolter, Timo L. Boelsums, Erwin Duizer, ..., Marion P. G. Koopmans
Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands; Center for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands; Internal medicine, department infectious diseases, Amsterdam UMC, Amsterdam, The Netherlands; Pandemic and Disaster Preparedness Center (PDPC), Delft, Rotterdam, the Netherlands; Division Fumigation and Disinfection, Eco Worldwide Solutions B.V., Werkendam, the Netherlands; Emergency Preparedness and Response Section, European Centre for Disease Prevention and Control, Stockholm, Sweden; Public Health Service Rotterdam-Rijnmond, Rotterdam, the Netherlands; KWR Water Research Institute, 3433 PE Nieuwegein, The Netherlands / Delft University of Technology, 2628 CN Delft, The Netherlands; Section Virology, Division Infectious Diseases and Immunology, Department Biomolecular Health Sciences, Faculty Veterinary Medicine, Utrecht University, Utrecht, the Netherlands; Regional office for Africa, World health organization, Brazzaville, Congo
The design comprised 62 overlapping primers generating ~400 bp amplicons in two pools. A third pool contained three L-segment amplicons targeting outbreak-specific SNPs to increase sensitivity for outbreak specimens. Random priming used 65°C for 5 min followed by 2 min snap cooling at 4°C. cDNA was synthesized with SuperScript IV (Invitrogen, Carlsbad, USA) at 23°C for 10 min,...
Sequenced by - organization
Erasmus MC
Consensus sequence software name
ARTIC field bioinformatics pipeline (https://github.com/artic-network/fieldbioinformatics)