PP_002YX4W.1

Display Name: Canada/PP_002YX4W.1/2025
Jonathan Audet, Karla Soriano, Anders Leung, David Safronetz & Jim Strong

Submission details

Submission ID
EBOV/VECTOR/C.porcellus-lab/COD/1976/Mayinga_NML
Submitting group
Date submitted
2025-06-25 21:30:13 UTC
Date released
2025-06-25 21:32:19 UTC

Data use terms

Data use terms
OPEN

Data Use Terms History

ChangedUserData Use Terms
2025-06-25 21:30jaudetOPEN
Data use terms URL

Sequencing

Amplicon PCR primer scheme
ARTIC 1.0
Amplicon size
400
Reference genome accession
NC_002549.1
Dehosting method
N/A
Depth of coverage
1000
Sequenced by - contact email
jonathan.audet@phac-aspc.gc.ca
Sequenced by - contact name
Jonathan Audet
Sequenced by - organization
PUBLIC HEALTH AGENCY OF CANADA
Sequencing date
2025-06-24
Sequencing instrument
MinION Mk1D
Sequencing protocol
RT-PCR Tiling amplicons using ARTIC EBOV primers. Library prep using Native Barcoding Kit (SQK-NBD114.24). Data analyzed using nf-ViralMutations (commit 5a6ba36; https://github.com/phac-nml/nf-ViralMutations).)

Authors

Author affiliations
Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada

INSDC

BioProject accession
BioSample accession
INSDC accession

Host

Cell line
Vero E6
Host health state
MB
Host name - scientific
Cavia porcellus
Is lab host
True
Passage number
2

Alignment and QC metrics

Completeness
98.01%
Length
18582
Total ambiguous nucs
0
Total deleted nucs
0
Total frame shifts
0
Total inserted nucs
0
Total SNPs
9
Total unknown nucs
0

Sample details

Earliest release date
2025-06-25
Collection country
Canada
Collection date
2025
Collection date (lower bound)
2025-01-01
Collection date (upper bound)
2025-06-25
Isolate name
Ebola virus/VECTOR/C.porcellus-lab/COD/1976/Mayinga_NML

Nucleotide mutations

Mutations called relative to the NC_002549.1 reference

Substitutions

  • A1003C
  • T4429C
  • C5053T
  • T6424C
  • C10420T
  • C10741A
  • G13053A
  • A13710G
  • A13968G
    • Deletions
    None
    Insertions
    None

    Amino acid mutations

    Mutations called relative to the NC_002549.1 reference

    Substitutions

    GP

  • GP:I129T

    • VP24

  • VP24:L26F
  • VP24:H133N

    • VP40

  • VP40:T192I

    • sGP

  • sGP:I129T

    • ssGP

  • ssGP:I129T
    • Deletions
    None
    Insertions
    None

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