PP_002YX4W.1

Display Name: Canada/PP_002YX4W.1/2025
Jonathan Audet, Karla Soriano, Anders Leung, David Safronetz & Jim Strong

Sample details

Collection date
2025
Country
Canada
Isolate name
Ebola virus/VECTOR/C.porcellus-lab/COD/1976/Mayinga_NML

Data use terms

Data use terms
OPEN

Data use terms history

ChangedUserData use terms
2025-06-25 21:30jaudetOPEN
Data use terms URL

Outbreak

Outbreak
Ebov-1976

Authors

Author affiliations
Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada

INSDC

INSDC accession
BioProject accession
BioSample accession
GCA accession

Host

Host name - scientific
Cavia porcellus
Host health state
MB
Is lab host
True
Cell line
Vero E6
Passage number
2

Sequencing

Sequencing date
2025-06-24
Amplicon PCR primer scheme
ARTIC 1.0
Amplicon size
400
Sequencing instrument
MinION Mk1D
Sequencing protocol
RT-PCR Tiling amplicons using ARTIC EBOV primers. Library prep using Native Barcoding Kit (SQK-NBD114.24). Data analyzed using nf-ViralMutations (commit 5a6ba36; https://github.com/phac-nml/nf-ViralMutations).)
Sequenced by - organization
PUBLIC HEALTH AGENCY OF CANADA
Sequenced by - contact name
Jonathan Audet
Sequenced by - contact email
jonathan.audet@phac-aspc.gc.ca
Dehosting method
N/A
Reference genome accession
NC_002549.1
Depth of coverage
1000

Alignment and QC metrics

Length
18582
Completeness
98.01%
# of SNPs
9
# of inserted bases
0
# of deleted bases
0
# of ambiguous bases
0
# of unknown bases
0
# of frame shifts
0
# of stop codons
0

Submission details

Submission ID
EBOV/VECTOR/C.porcellus-lab/COD/1976/Mayinga_NML
Submitting group
Date submitted
2025-06-25 21:30:13 UTC
Date released
2025-06-25 21:32:19 UTC
Earliest release date
2025-06-25

Nucleotide mutations

Mutations called relative to the NC_002549.1 reference

Substitutions

  • A1003C
  • T4429C
  • C5053T
  • T6424C
  • C10420T
  • C10741A
  • G13053A
  • A13710G
  • A13968G
    • Deletions
    None
    Insertions
    None

    Amino acid mutations

    Mutations called relative to the NC_002549.1 reference

    Substitutions

    GP

  • GP:I129T

    • VP24

  • VP24:L26F
  • VP24:H133N

    • VP40

  • VP40:T192I

    • sGP

  • sGP:I129T

    • ssGP

  • ssGP:I129T
    • Deletions
    None
    Insertions
    None

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