Rainer Gosert, Karoline Leuzinger, Alexander Kuznetsov & Richard Neher
Clinical Virology, Laboratory Medicine, University Hospital Basel, Basel, Switzerland; Clinical Virology, Laboratory Medicine, University Hospital Basel, Basel, Switzerland, Transplantation and Clinical Virology, Department of Biomedicine, University of Basel, Basel, Switzerland; Biozentrum, University of Basel, Basel, Switzerland
500 bp primers were designed using PRIMAL tool. Additionally, 2000bp-NAT primers and 1000bp-RSV-A and RSV-B subgroup-specific NAT primers were used from Wang et al. (doi: 10.1016/j.jviromet.2021.114335), and used in 2 separate primer pools.
Amplicon size
500bp, 1000bp, 2000bp
Sequencing instrument
Illumina MiniSeq [OBI:0003114]
Sequencing protocol
Genomes were generated through amplicon sequencing of 500, 1000, and 2000 bp amplicons in separate primer pools. Libraries were created using KAPA HyperPrep Kit, and sequence data was produced using Illumina MiniSeq