Display Name: Ireland/PP_0015L11.1/2025-02-04
Michael Carr, Laura Fahey, Jonathan Dean, Daniel Hare & Cillian F. De Gascun

Sample details

Collection date
2025-02-04
Country
Ireland
Admin level 1
Ireland
Admin level 2
na

Data use terms

Data use terms
OPEN

Clade & Lineage

Clade
IIb
Outbreak
hMPXV-1
Lineage
F.4.1

INSDC

INSDC accession
BioProject accession
BioSample accession
GCA accession

Host

Host name - scientific
Homo sapien

Sequencing

Sequencing date
2025-02-05
Sequencing instrument
Oxford Nanopore GridION
Sequencing protocol
Genomes were generated through amplicon sequencing of 2500 bp amplicons with Molecular Biology Systems primers. The ARTIC fieldbioinformatics pipeline was used to generate consensus genomes. Analysis was repeated twice, once with the top of reference fasta cut off and once with the bottom of consensus fasta cut off, in order to overcome tandem repeats at either end of the genome.
Depth of coverage
196

Alignment and QC metrics

Length
196764
Completeness
96.46%
# of SNPs
79
# of inserted bases
0
# of deleted bases
38
# of ambiguous bases
0
# of unknown bases
6583
# of frame shifts
0
# of stop codons
0

Submission details

Submission ID
hMpxV/Ireland/NVRL-GIRL00011475/2025
Submitting group
Date submitted
2025-05-06 12:10:18 UTC
Date released
2025-05-06 12:11:21 UTC
Earliest release date
2025-05-06

Nucleotide mutations

Mutations called relative to the NC_063383.1 reference

Substitutions

  • G1262A
  • G3111A
  • G3522A
  • C3818T
  • C7771T
  • G7862A
  • G14000T
  • G14118A
  • G15428A
  • A18769G
  • C20453T
  • G21394A
  • G21723A
  • C23564T
  • G25661A
  • T28175C
  • G30367A
  • G31053A
  • Deletions
    22197-22199, 133177-133186, 150586-150602, 196610-196617
    Insertions
    None

    Amino acid mutations

    Mutations called relative to the NC_063383.1 reference

    Substitutions

    OPG001

  • OPG001:S105L
  • OPG003

  • OPG003:D264N
  • OPG019

  • OPG019:D95N
  • OPG025

  • OPG025:H384Y
  • OPG025:A423D
  • OPG037

  • OPG037:S347F
  • OPG047

  • OPG047:R48C
  • OPG053

  • OPG053:P78S
  • OPG056

  • OPG056:E125K
  • OPG057

  • OPG057:E353K
  • OPG071

  • OPG071:L108F
  • OPG072

  • OPG072:D56N
  • OPG092

  • OPG092:D196N
  • OPG093

  • OPG093:S30L
  • OPG093:D88N
  • OPG094

  • OPG094:M142I
  • OPG098

  • OPG098:E162K
  • OPG109

  • OPG109:H740Y
  • OPG113

  • OPG113:H218Y
  • OPG118

  • OPG118:K606E
  • OPG136

  • OPG136:D98N
  • OPG139

  • OPG139:A17T
  • OPG145

  • OPG145:E62K
  • OPG145:E435K
  • OPG148

  • OPG148:G312S
  • OPG150

  • OPG150:S307L
  • OPG175

  • OPG175:S29L
  • OPG176

  • OPG176:H221Y
  • OPG193

  • OPG193:L263F
  • OPG209

  • OPG209:S30L
  • OPG210

  • OPG210:D209N
  • OPG210:P722S
  • OPG210:M1741I
  • Deletions
    OPG037:81
    Insertions
    None

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