Ireland/PP_0015L03.1/2025-01-13
Michael Carr, Laura Fahey, Jonathan Dean, Daniel Hare & Cillian F. De Gascun

National Virus Reference Laboratory, UCD Ireland

Sample details

Collection date
2025-01-13
Sampling location
Ireland (na, Ireland)

Data use terms

Data use terms
OPEN

Data use terms history

ChangedUserData use terms
2025-05-06 12:10lfaheyOPEN

Clade & Lineage

Clade
IIb
Outbreak
sh2017
Lineage
F.4.1

INSDC

INSDC accession
BioProject accession
BioSample accession
GCA accession

Sequencing

Sequencing date
2025-02-05
Sequencing instrument
Oxford Nanopore GridION
Sequencing protocol
Genomes were generated through amplicon sequencing of 2500 bp amplicons with Molecular Biology Systems primers. The ARTIC fieldbioinformatics pipeline was used to generate consensus genomes. Analysis was repeated twice, once with the top of reference fasta cut off and once with the bottom of consensus fasta cut off, in order to overcome tandem repeats at either end of the genome.
Depth of coverage
215

Submission details

Submission ID
hMpxV/Ireland/NVRL-GIRL00005334/2025
Submitting group
Date submitted
2025-05-06 12:10:18 UTC
Date released
2025-05-06 12:11:21 UTC
Earliest release date
2025-05-06

Alignment and QC

Length
196764 (98%)
# of SNPs
77
# of deleted bases
38
# of unknown bases
3623

Nucleotide mutations

Mutations called relative to the NC_063383.1 reference

Substitutions

  • G1262A
  • G3111A
  • G3522A
  • C3818T
  • C7771T
  • G14000T
  • G14118A
  • G15428A
  • A18769G
  • C20453T
  • G21394A
  • G21723A
  • C23564T
  • G25661A
  • T28175C
  • G30367A
  • G31053A
  • C34277T
Deletions

22197-22199, 133177-133186, 150586-150602, 196610-196617
Insertions
N/A

Amino acid substitutions

Substitutions called relative to the NC_063383.1 reference

Substitutions

OPG001

  • OPG001:S105L

OPG003

  • OPG003:D264N

OPG025

  • OPG025:H384Y
  • OPG025:A423D

OPG037

  • OPG037:S347F

OPG047

  • OPG047:R48C

OPG053

  • OPG053:P78S

OPG056

  • OPG056:E125K

OPG057

  • OPG057:E353K

OPG071

  • OPG071:L108F

OPG072

  • OPG072:D56N

OPG092

  • OPG092:D196N

OPG093

  • OPG093:S30L
  • OPG093:D88N

OPG094

  • OPG094:M142I

OPG098

  • OPG098:E162K

OPG109

  • OPG109:H740Y

OPG113

  • OPG113:H218Y

OPG118

  • OPG118:K606E

OPG136

  • OPG136:D98N

OPG139

  • OPG139:A17T

OPG145

  • OPG145:E62K
  • OPG145:E435K

OPG148

  • OPG148:G312S

OPG150

  • OPG150:S307L

OPG175

  • OPG175:S29L

OPG176

  • OPG176:H221Y

OPG193

  • OPG193:L263F

OPG209

  • OPG209:S30L

OPG210

  • OPG210:D209N
  • OPG210:P722S
  • OPG210:M1741I
Deletions

OPG037

OPG037:81
Insertions
N/A

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