PP_000SLJS.1

USA/EH43L-8-29-Cqp/2019-08-29
Nicole M. Feriancek, Mallery I. Breban, Ellie Bourgikos, Angela Bransfield, Philip M. Armstrong, Chantal B. F. Vogels & Nathan D. Grubaugh

Grubaugh Lab, Yale University, CT, USA

Sample details

Collection date
2019-08-29
Latitude
41.26405
Longitude
-72.87785
Sampling location
USA (New Haven, Connecticut)
City
East Haven
Isolate name
EH43L-8-29-Cqp

Data use terms

Data use terms
OPEN
Data use terms URL

Lineage

Lineage
1A

INSDC

INSDC accession
BioProject accession
BioSample accession
GCA accession

Sampling

Sample type
pooled blended mosquitoes
Purpose of sampling
surveillance for arboviruses
Collection device
Mosquitos trapped using Light Trap
Collection method
mosquito trapping

Specimen processing

Specimen processing details
Sample consisted of 50 whole, blended mosquitoes from a given trap pooled in groups of up to 50 by species and date

Host

Host
Is lab host
False

Sequencing

Sequencing date
2024-02-19
Amplicon PCR primer scheme
WNVUS1
Amplicon size
400bp
Sequencing protocol
COVIDSeq protocol (primers switched)
Sequenced by - organization
Grubaugh Lab, Yale University, CT, USA
Sequenced by - contact name
Nicole M Feriancek
Raw sequence data processing method
iVar v1.3.1
Consensus sequence software name
iVar
Consensus sequence software version
v1.3.1
Depth of coverage
10
Quality control method name
iVar trim
Quality control method version
v1.3.1

Submission details

Submission ID
Yale-WNV0627
Submitting group
Date submitted
2024-11-06 12:51:04 UTC
Date released
2024-11-06 12:55:45 UTC
Earliest release date
2024-11-06

Files

Annotations

Cited in

Alignment and QC

Length
10914 (99%)
# of SNPs
87
# of deleted bases
3
# of ambiguous bases
3

Nucleotide mutations

Mutations called relative to the NC_009942.1 reference

Substitutions
  • C52T
  • C522T
  • A630G
  • T1033C
  • C1119T
  • A1401G
  • T1442C
  • C1465T
  • T1965C
  • T1983C
  • A2097C
  • T2154C
  • C2371T
  • C2466T
  • T3138C
  • C3324T
  • T3444G
  • C3504T
Deletions
10468-10470
Insertions
N/A

Amino acid substitutions

Substitutions called relative to the NC_009942.1 reference

Substitutions

NS2A

  • NS2A:T52I
  • NS2A:L95F
  • NS2A:I183M

NS3

  • NS3:S334T

NS4B

  • NS4B:S14I

env

  • env:V159A
  • env:L167F
Deletions
N/A
Insertions
N/A

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