PP_000RGTE.1

Display Name: USA/PP_000RGTE.1/2016-07-26
Feriancek, Nicole M.; Breban, Mallery I.; Bourgikos, Ellie; Bransfield, Angela; Armstrong, Philip M.; Vogels, Chantal B.F.; Grubaugh, Nathan D.

Submission details

Submission ID
Yale-WNV0002
Submitting group
Grubaugh Lab
Date submitted
2024-09-30 12:24:47 UTC
Date released
2024-09-30 12:26:57 UTC

Data use terms

Data use terms
OPEN

Data Use Terms History

ChangedUserData Use Terms
2024-09-30 12:24aparkerRESTRICTED until 2024-10-01
Data use terms URL
https://pathoplexus.org/about/terms-of-use/open-data

Sequencing

Amplicon PCR primer scheme
WNVUS1
Amplicon size
400bp
Consensus sequence software name
iVar
Consensus sequence software version
v1.3.1
Depth of coverage
10
Quality control method name
iVar trim
Quality control method version
v1.3.1
Raw sequence data processing method
iVar v1.3.1
Sequenced by - contact name
Nicole M Feriancek
Sequenced by
Grubaugh Lab, Yale University, CT, USA
Sequencing date
2024-01-09
Sequencing protocol
COVIDSeq protocol (primers switched)

Authors

Author affiliations
Grubaugh Lab, Yale University, CT, USA

INSDC

Bioproject accession
PRJEB80643
Biosample accession
SAMEA116120071
INSDC accession
OZ187637.1

Sampling

Collection device
Mosquitos trapped using Gravid Trap
Collection method
mosquito trapping
Purpose of sampling
surveillance for arboviruses
Sample type
pooled blended mosquitoes

Alignment states and QC metrics

Completeness
98.98%
Length
11029
Total ambiguous nucs
1
Total deleted nucs
0
Total frame shifts
0
Total inserted nucs
0
Total SNPs
65
Total stop codons
0
Total unknown nucs
112

Sample details

Collection subdivision level 1
Connecticut
Collection subdivision level 2
Fairfield
Collection city
Bridgeport
Collection country
USA
Latitude
41.2111
Longitude
-73.17992
Collection date
2016-07-26
Isolate name
BP39G-7-26-Cxp-2

Host

Host name scientific
Culex pipiens
Is lab host
False

Lineage

Lineage
1A

Specimen processing

Specimen processing details
Sample consisted of 50 whole, blended mosquitoes from a given trap pooled in groups of up to 50 by species and date

Nucleotide mutations

Substitutions

  • C93T
  • G237A
  • T309C
  • C435T
  • A630G
  • C687T
  • C693T
  • T1442C
  • C1465T
  • C1560T
  • C1599T
  • C1953T
  • A2313G
  • T2400C
  • C2466T
  • G2610A
  • C2787T
  • C3348T
    • Deletions
    None
    Insertions
    None

    Amino acid mutations

    Substitutions

    NS1

  • NS1:Q347R

    • NS2A

  • NS2A:I187V

    • NS3

  • NS3:E180D

    • NS4B

  • NS4B:V23I
  • NS4B:M29I

    • env

  • env:V159A
  • env:L167F
    • Deletions
    None
    Insertions
    None