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PP_006XDJH.6	PP_006XDJH	6	MC202601-001-NP	false	jaudet	SP_NML_PHAC	397	1780087417	2026-05-29	1780087424	2026-05-29	RESTRICTED	2027-05-17		https://pathoplexus.org/about/terms-of-use/restricted-data	LATEST_VERSION		1				Nasopharynx		Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada; Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada; Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada; BC Center for Disease Control Public Health Laboratory; BC Center for Disease Control Public Health Laboratory; BC Center for Disease Control Public Health Laboratory; BC Center for Disease Control Public Health Laboratory; BC Center for Disease Control Public Health Laboratory; BC Center for Disease Control Public Health Laboratory; BC Center for Disease Control Public Health Laboratory; BC Center for Disease Control Public Health Laboratory	Audet, Jonathan; Chan, Mable; Safronetz, Dave; Tyson, John; Lee, Tracy; Cheung, Branco; Prystajecky, Natalie; Eckbo, Eric; Morshed, Muhammad; Jassem, Agatha; Hoang, Linda						Swab	Swab		0.9303565985979885	0.864069735766821	0.8508818813468734											Canada/PP_006XDJH.6/2026-05-15	2026-05-17																Canada										human	Homo sapiens			9606													6105	3172	1766																						2026-05-15	2026-05-15	2026-05-15							2026-05-17	This sequence is specific to Illumina generated sequence data, but Oxford Nanopore sequence data was also generated and showed only 2 additional mixed base positions across all segments.	The RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen). The reverse transcription was performed using SuperScript IV (ThermoFisher) following manufacturers recommendations, using 8 µL of RNA extract. A two pool 400bp tiled amplicon scheme was designed using PrimalScheme with PP_006WBLH.2 as the reference genome ( https://labs.primalscheme.com/detail/bccdc-andv-2026/400/v1.0.0/ ). The PCR amplicon generation was carried out using Q5 polymerase (NEB) with 2.5 µL of cDNA template into two 25 µL reactions, each using 2ul of one of the two 10uM primer pool stocks. The cycling conditions were 98°C for 2 minutes, 35 cycles of 98°C for 30 seconds and 65°C for 5 minutes. The PCR products were pooled and sequencing libraries generated with either a modified Illumina DNAPrep (PMID: 38315007) or Oxford Nanopore Technologies Microbial Amplicon Barcoding Kit 24 V14 and sequenced on either a MiSeq or MinION Mk1D using a R10.4.1 Flongle flow cell. Consensus viral segment sequences were generated using amplicon-nf (v1.0.0dev).								0	0	1	0	0	2	0	0	0	0	0	6	361	183	114	0	0	0	0	0	169