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PP_006XDJH.2	PP_006XDJH	2	HAN260146	false	jaudet	SP_NML_PHAC	397	1779056576	2026-05-17	1779056583	2026-05-17	RESTRICTED	2027-05-17		https://pathoplexus.org/about/terms-of-use/restricted-data	REVISED		1						Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada	Audet, Jonathan; Chan, Mable; Safronetz, Dave									0.8450167631819567	0.724325796785617	0.8546231961517905											Canada/PP_006XDJH.2/2026-05-15	2026-05-17																Canada										human	Homo sapiens			9606													6367	3565	1710																						2026-05-15	2026-05-15	2026-05-15									The RNA was extracted using the Qiagen viral RNA kit. The reverse transcription was performed using SuperScript IV (ThermoFisher) following the manufacturer’s recommendation, using 8 µL of RNA. The PCR step was carried out using Phusion U Multiplex Master Mix (ThermoFisher) with 2 µL of template in 25 µL reactions. The cycling conditions were 98°C for 2 minutes, 45 cycles of 98°C for 30 seconds, 65°C for 5 minutes, and a final extension at 72°C for 10 minutes. The PCR products were bead-purified using 0.8× MagMAX PureBind magnetic beads (ThermoFisher), washed twice with 80% ethanol, and eluted in 20 µL of 1 mM Tris pH 8.0 (ThermoFisher). The pools were quantified using a Qubit Broad Range dsDNA 1× kit (ThermoFisher) and combined at equal proportions where possible. The pooled amplicons were prepared using the Native Barcoding Kit V14 (SQK-NBD114.24; Oxford Nanopore Technologies) and sequenced on a MinION Mk1D device using  a FLO-MIN114 flow cell. The data was analyzed using nf-ViralMutations (github.com/phac-nml/nf-ViralMutations; commit 8675768).								0	0	0	0	0	2	0	0	0	0	0	6	333	151	114	0	0	0	822	906	107